| 27031 Microbiology and Fermentation Technology |
| Danish title: Mikrobiologi og fermenteringsteknologi |
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Language: Danish ECTS-creditpoints: 12,5, External examination.
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Class schedule:
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F5 og juni |
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Exam schedule:
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F5-A (jun 07 2002), E5-B (dec 21 2001)
Plus one independent exercise in the 13 week period and one independent exercise in June |
| Recommended semester: 4th -7th semester |
| Scope and form: Lectures, group exercises and laboratory exercises |
Evaluation: Project report
2 independent exercise and a written exam after 13-week period (13 point scale) and each counts 50% of the mark. Both parts must obtain the character 5. The laboratory exercises are evaluated by reporting/oral presentaiton. |
| Examination: 13-scale |
| Prerequisites: 27000 , 27001, 28120, 36100 (gerne samtidig) |
| Preferred prerequisites: 27021 |
| No credit points with: 30001 |
| Aim: The students should acquire a fundamental knowledge within microbial diversity and obtain a general knowledge about the methods and principles that are of importance for design of fermentation processes. The students should achieve an understanding of how the design of fermentation processes interacts with microbial diversity and molecular biology, and they should be able to use this knowledge both in connection with industrial production and in research. The students should in addition achieve experience in experimental work within microbiology and fermentation technology, and thereby get practical experience in the subjects treated in the theoretical part of the course. |
Contents: Theory
. General microbiology: The microbial diversity is covered by discussing the phylogenetic, taxonomic and metabolic similarities and differencies between microorganisms belonging to bacteria, archae and fungi. There is focus on biotechnologically important microorganisms. Microbial ecology.
. Microrganisms for industrial use: Preservation of microorganisms. Isolation from natural habitants and identification of microorganisms, mutagenesis, screening, gene shuffling, directed evolution, functional genomics, metabolic engineering.
. The bioreactor and general aspects of bioprocesses: Preparation of inoculum, medium, design of bioreactors, sterilisation, analysis and control of bioprocesses. Downstream processing and economy.
. Proceskinetic and masstransfer: kinetics of microbial processes, stoichiometrics and mass transfer. Aeration and agitation. Mass balances. Process integration.
. Biotechnology processes: There will be given two independent exercises, where the students will work on the theory presented in the lectures, treating a specific bioprocess in depth.
Laboratory exercises
Aspects to be treated: Aseptic technique. Preservation of microorganisms, propagation and inoculum. Cultivation in fermenters; batch and chemostat. Practical experience in setting up a bioreactor. Growth characteristics and mass balances based on experimental data. Isolation, identification and characterisation of microorganisms. |
| Contact: Lisbeth Olsson, building 223, (+45) 4525 2677, lisbeth.olsson@biocentrum.dtu.dk |
| Department: 027 BioCentrum-DTU |
| Updated: 11-02-2002 |
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