| 27302 Experimental Biochemistry |
| Danish title: Eksperimentel biokemi |
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Language: English ECTS-creditpoints: 5
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| Type: , course at phd level, open university |
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Class schedule:
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januar
Start 7.01.02 |
| Scope and form: 3 week period - whole days. |
Evaluation: Oral presentation and approval of reports
Projects will be evaluated during the course. |
| Examination: Pass/fail |
| Previous course: 25261 |
| Prerequisites: 25151, 25111, 27021 |
| Preferred prerequisites: 25221, 27301 first time E02 |
| No credit points with: 25261 |
| Participant limitation: Max. 16 |
| Aim: To introduce advanced methodology in biochemical research. |
| Contents: Biochemical techniques: Extraction methods, preparative column chromatography, incl. FPLC and affinity-chromatography. Analytical methods for characterisation of biomacromolecules, incl. gel electrophoresis, immunelectrophoresis, isoelectric focusing, ELISA-techniques, immunoblotting, HPLC, FPLC and GC. Metabolic studies: Purification of subcellular organelles using preparative ultracentrifugation, use of marker enzymes. Purification of peripheral neutrophils from man and peritoneal neutrophils from rats. Application of radioisotopes in metabolic studies. Image processing and classification of complex biochemical data. Isolation, separation and characterisation of lipoproteins. |
| Contact: Klaus Bukhave, building 224, (+45) 4525 2739, klaus.bukhave@biocentrum.dtu.dk |
| Department: 027 BioCentrum-DTU |
| Keywords: Biological macromolecules, Blood cells, Affinity chromatography, Animal experiments, Neural nets |
| Updated: 13-12-2001 |
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