| 27405 Metabolic Engineering and Functional Genomics |
| One week in June every second year this course is offered as a Ph.D. course. Furthermore a different version of the course is offered in collaboration with Lund University in connection with the Øresund Summer University. |
| Danish title: Metabolic Engineering og Functional Genomics |
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Language: English ECTS-creditpoints: 10, External examination.
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| Type: , course at phd level, open university |
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Exam schedule:
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E4-A (dec 18 2001), F4-A (jun 04 2002) |
| Scope and form: Lectures, group exercises, seminars, problem solving. |
Evaluation: Oral exam
Oral exam. Approval of compulsory activities is a prerequisite for the exam. (13-scale). |
| Examination: 13-scale |
| Previous course: 30205 |
| Prerequisites: 27031, 28120 |
| Preferred prerequisites: 27011 |
| Aim: The aim of the course is to give the students a fundamental understanding of the interplay between the many different intracellular reactions, and especially how the fluxes through the different pathways are regulated; with special focus on pathways leading to industrially relevant products like primary metabolites, antibiotics, industrial enzymes, and pharmaceutical proteins. A central aspect of the course is to identify the optimal strategy for introducing directed genetic changes in the microorganisms with the aim of obtaining better production strains. Analysis of the interaction between different cellular reactions is a central element in the course, and tools from functional genomics will therefore be describes. This will involve tools like DNA arrays, proteomics and bioinformatics. |
| Contents: The course gives an overview of the different elements of metabolic engineering with a number of examples on how directed genetic modification using rDNA technology have been introduced with the aim of obtaining better strains for production of different compounds in the bioindustry. There is especially focus on the different tools of metabolic engineering, and the course covers the following topics: Introduction to metabolic engineering. Overview of biochemical pathways. Energetics. Regulation of pathways. Examples of metabolic engineering. Identification of gross measurement errors. Metabolic flux analysis: Theory and applications. Application of 13C-isotopes for quantification of metabolic fluxes. Metabolic control analysis: Theory and Application. Application of metabolic control analysis on complex reaction network. Pathway synthesis. Thermodynamic analysis of biochemical reactions. Classical and non-equilibrium thermodynamics of biochemical reactions. Genomics. Introduction of directed genetic modifications. Recombination. Molecular biological tools in metabolic engineering. Whole genome transcription analysis using DNA arrays. Proteomics. 2D-electrophoresis as a tool for analysis of cellular function. Bioinformatics. Clusteranalysis for evaluation of DNA array data. In the description there is especially focus on a few processes, e.g. amino acid production by Corynebacteria, production of antibiotics, lactic acid fermentation, and industrial applications of the yeast Saccharomyces cerevisiae. |
| Contact: Jens Nielsen, building 223, (+45) 4525 2696, jens.nielsen@biocentrum.dtu.dk |
| Department: 027 BioCentrum-DTU |
| Updated: 13-06-2001 |
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