| 27733 Separation Methods for Biological Compounds |
| Danish title: Metoder til separation af biologisk materiale |
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Language: English ECTS-creditpoints: 5, External examination.
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| Type: , open university, food engineering |
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Exam schedule:
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F3-A (maj 31 2002), E3-A (dec 15 2001) |
| Recommended semester: 6th or 7th semester |
| Scope and form: Lectures and laboratory work two afternoons per week during the last half of the term. |
| Evaluation: Written exam and approval of reports
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| Examination: 13-scale |
| Previous course: 25780 |
| No credit points with: 25780 |
| Participant limitation: Max. 16 |
| Aim: To give a thorough theoretical and practical introduction to newer methods of separation of biomolecules, including macromlecules of improtance for Food production and isolation and characterisation of subcellular membrane fractions |
| Contents: Lectures on the theoretical basis of separation and detection systems. Theoretical and instrumental introduction to HPLC, GC, capillary electrophoresis, mass spectrometry, two dimensional electrophoresis,centrifugation, amino acid analysis, and protein sequencing. The experimental work will include sample preparation, column packing test, evaluation of columns and separations of amino acids, proteins, vitamins, lipids and membrane fractions from different types of tissue. The utility of the methods will be discussed with special attention to their compatibility with the stability of biological material. |
| Remarks: Students following the Food Science and Technology education has priority. The course is open for other students if there are vacancies. |
Contact: Vibeke Barkholt, building 224, (+45) 4525 2751, vibeke.barkholt@biocentrum.dtu.dk
Huiling Mu, building 224, (+45) 4525 2744, huiling.mu@biocentrum.dtu.dk |
| Department: 027 BioCentrum-DTU |
| Keywords: Chromatography, centrifugation, HPLC, GC |
| Updated: 15-03-2001 |
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