| 27802 Metabolic engineering |
| Danish title: Metabolic engineering |
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Language: English ECTS-creditpoints: 4,8
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| Type: course at phd level |
| Program: Chemical Engineering |
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Class schedule:
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11-18 May 2002 |
| Scope and form: Lectures, seminars by students and exercises |
Evaluation: Project report
Written examination and test/homework |
| Examination: Pass/fail |
| Aim: The aim of the course is to give the students a fundamental understanding of the interplay between the many different interacellular reactions, and especially how the fluxes through the different pathways are regulated; with special focus on pathways leading to industrially relevant products like primary metabolites, antibiotics, industrial enzymes, and pharmaceutical proteins. A central aspect of the course is to identify the optimal strategy for introducing directed genetic chages in the microorganisms with the aim of optaining better production strains. |
| Contents: The course gives an overview of the diffrent elements of metabolic engineering with a number of examples on how directed genetic modification using rDNA technology have been introduced with the aim og obtaining better strains for production of different compounds in the bioindustry. There is especially focus on the different tools of metabolic engineering , and the course covers the following topics: Introduction to metabolic engineering. Overview of biochemical pathways. Energetics. Regulation of pathways. Examples of metabolic engineering. Identification of gross measurement errors. Metabolic flux analysis: Theory and application. Application of 13C-isotopes for quantification of metabolic fluxes. Metabolic control analysis: Theory and Application. Apllication of metabolic control analysis on complex reaction network. Pathway synthesis. Thermodynamic analysis of biochemical reation. Classical and non-equilibrium thermodynamics of biochemical reactions. In the description there is especially focus on a few processes, e.g. amino acid production by Corynebacteria, production of antibiotics, lactic acid fermentation, and industrial application of the yeast Saccharmyces cerevisisae. |
| Department: 027 BioCentrum-DTU |
| Keywords: metabolic engineering, metabolic flux analysis, metalbolic control analysis, pathway synthesis, thermodynamic analysis |
| Signup: Hos lfreren/At the teacher |
| Updated: 12-02-2002 |
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