| 27804 Lipid biochemistry and metabolism related to biotechnology |
| An introduction to advanced analytical methods and theory of lipid biochemistry |
| Danish title: Lipid biokemi og metabolisme i relation til bioteknologi |
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Language: English ECTS-creditpoints: 5
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| Type: course at phd level |
| Program: Chemical Engineering |
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Class schedule:
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2 weeks in even years
27. May - 7. June 2002 9.00 - 17.00 |
| Scope and form: Lectures and excersis |
| Examination: Pass/fail |
| Participant limitation: Min. 6 Max. 12 |
| Contents: : The objective of the course is to introduce the participants to lipid biochemistry and advanced lipid analytical methods. The following areas will be covered: modification of lipids, lipid and lipoprotein metabolism, biological membranes, lipid oxidation and antioxidants, different lipid analytical methods (GLC, HPLC, GC-MS and LC-MS) and isotope techniques. Structured triacylglycerols are produced by continuous and batch interesterification, HPLC is used for monitoring the products and LC / MS is used for the identification of acylglycerols. Interesterification products may also be characterized by regiospecific degradation followed by chiral phase HPLC and GLC. Absorption of structured lipids and different tocopherols are studied. Incorporation of dietary fatty acids and antioxidants into cell membranes are studied using cell fractionation techniques followed by lipid and antioxidant analysis. Isotope techniques are illustrated by instillation of radio-labeled lipids into "tied-off loops" of rat jejunum, and the radioactivity in the complex lipid classes are measured. Stable isotope technique in the tracing of the lipid metabolism is demonstrated by GC-C-IRMS. |
| Contact: Huiling Mu, building 224, (+45) 4525 2744, huiling.mu@biocentrum.dtu.dk |
| Department: 027 BioCentrum-DTU |
| Keywords: lipid bichemistry, metabolism, GLC, HPLC, GC-MS |
| Signup: Hos lfreren/At the teacher |
| Updated: 07-09-2001 |
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