| 27941 Introduction to Biotechniques |
| Semicompulsory course at Diplom-kemi |
| Danish title: Bioteknisk kemi og -metodik |
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Language: Danish ECTS-creditpoints: 10, External examination.
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Class schedule:
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E5 og E3-B |
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Exam schedule:
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F5-B (jun 08 2002), E5-B (dec 21 2001)
Two hour written examination, counts 50% of the score.
Evaluation of projekt counts 50% of the score. |
| Recommended semester: 4th semester |
| Scope and form: Classroom lectures, colloquies and group exercises. |
Evaluation: Written exam and approval of reports
Approval of laboratory exercisis are compulsory before examination. |
| Examination: 13-scale |
| Previous course: 91541 |
| Compulsory: 27931 |
| Prerequisites: 26470 |
| Aim: The aim of the course is to broaden the knowledge of unicellular organisms structure, morphology and physiology in addition to the work with biological materials and systems. To give a knowledge of Good Laboratory Practices(GXP) for microbiological and biochemical work. To obtain general skills of biomethodology. |
Contents: Biochemical part : metabolism of carbohydrates, lipids and amino acids. Enzymes kinetics. Microbiological part : Techniques of culturing, analytical use of microorganisms, fermentation kinetics and -techniques (batch and chemostat). Sterilization. Biomethodology : GLP / GXP for biological laboratories. Separation techniques for biological molecules. Chromatograhic and electrophoretic techniques. Enzymatic and immunological techniques. Methods of detection for different groups of molecules. Litterature critics.
The practical part of the course contains : Microbiology : Qualitative and quantitative detection of microorganisms. Microbiological assays. Fermentations. Biochemistry : Enzymatic analysis (end-point and kinetics). Purification of enzymes, e.g. by precipitation, chromatography and electrophoresis. Characterization of enzymes. Immunology.
The project concerns a description and critisism of a biochemical or microbiological analysis method, based on a scientific article. |
Remarks: This course is part of a group of semi-compulsory courses consisting of 27941 and 27961.
The course rates 10 ECTS points in E2001
From F2002 onwards the course rates 12,5 ECTS points. The 2,5 ECTS points are placed in the 3-week period in january or june. |
| Contact: Marianne Kemner, building 378, (+45) 4525 5492, marianne.kemner@biocentrum.dtu.dk |
| Department: 027 BioCentrum-DTU |
| Keywords: Microbiology, Enzym kinetics, Isolation and analysis of protein, Fermentation |
| Updated: 25-04-2001 |
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