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25261 Experimental Biochemistry 2
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Danish title: Eksperimentel biokemi 2
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| Type: Å, Language: EEE |
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Credit points:
5 point |
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| | | Previous course: C2561 |
| Offered by:
Department of Biochemistry and Nutrition
(B&E) |
| No credit points with: C2561 |
| Prerequisite: 25151/C2551.25111/C2511 |
| Desirable: 25221/C2521,25231/C2531 |
| Recommended semester:
5th semester |
| Limitation: Max. 16 |
| Examination:
Approval of exercises.
(
pass/fail
) |
| Contact person: |
K. Bukhave, B&E, Building 224, Tel. +45 4525 2739 |
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| Aim: To introduce advanced methodology in biochemical research. |
| Contents: Biochemical techniques: Extraction methods, preparative column chromatography, incl. FPLC and affinity-chromatography. Analytical methods for characterization of biomacromolecules, incl. gel electrophoresis, immunelectrophoresis, isoelectric focusing, ELISA-techniques, immunblotting, HPLC, FPLC and GC. Metabolic studies: Purification of subcellular organelles using preparative ultracentrifugation, use of marker enzymes. Purification of peripheral neutrophils from man and peritoneal neutrophils from rats. Application of radioisotopes in metabolic studies. Image processing and classification of complex biochemical data. Isolation, separation and characterization of lipoproteins. |
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