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30206 Downstream Processing of Biotechnological Products
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Danish title: Oprensningsprocesser
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| Type: Å, Language: EEE |
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Credit points:
5 point |
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| Offered by:
Department of Biotechnology
(IBT) |
| Prerequisite: C3201,C3600/36100.30001 |
| Recommended semester:
7th semester |
| Scope and form: 2 lecture modules including problem solving per week. |
| Examination:
Written exam
(13 point scale
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| Contact person: |
Owen R.T. Thomas, IBT, Building 223, Tel. +45 4525 2703 |
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| Aim: This course will provide the student with a comprehensive knowledge of the practices employed in the recovery of industrially important biomolecules (e.g. organic acids, amino acids, antibiotics, peptide hormones, enzymes, therapeutic proteins, antibodies, vaccines) typically produced by fermentative (upstream) processes. |
| Contents: General process issues. Process synthesis and interactions. Unit operations and equipment: Cell removal/recovery, liquid-liquid extraction and solvent recovery, whole broth extractions, crystallisation, drying, supercritical fluid extraction; aqueous two liquid phase separations, cell disruption, precipitation and flocculation processes, centrifugation and industrial centrifuges; convention filtration and membrane separations (microporous filtration, ultrafiltration, reverse osmosis); batch adsorption/desorption operations; high resolution chromatographic techniques (ion exchange, gel permeation, hydrophobic interaction, affinity and pseudoaffinity adsorption, reverse phase HPLC). Future directions in downstream processing: New science and engineering, new products and prospects. |
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