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25780 Separation Methods for Biological Compounds
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Danish title: Separationsmetoder for biologiske materialer
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| Type: L, Å, Language: E |
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Credit points:
5 point |
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| | | Previous course: C2580 |
| Offered by:
Department of Biochemistry and Nutrition
(BE) |
| No credit points with: C2580 |
| Recommended semester:
7th - 9th semester |
| Limitation: Max. 16 |
| Scope and form: Lectures and colloquia. Laboratory work two afternoons per week during the last half of the term. |
| Examination:
Approval of compulsory activities is a prerequisite for taking part in the examination. Written exam
(13 point scale
) |
| Contact person: |
Vibeke Barkholt, BE, Building 224, Tel. +45 4525 2751 |
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| Contents: Lectures on the theoretical basis of separation and detection systems. Theoretical and instrumental introduction to HPLC, GC, capillary electrophoresis, mass spectrometry, two dimensional electrophoresis,centrifugation, amino acid analysis, and protein sequencing. The experimental work will include sample preparation, column packing test, and evaluation of columns and separations of amino acids, proteins, vitamins and lipids. The utility of the methods will be judged with special attention to their compatibility with the stability of biological material. |
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