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Archive 1999/2000

25002 Experimental Biological Chemistry Danish title: Eksperimentel biologisk kemi Language: Danish Credit points: 2,5 Type:  Open University Language: Danish Previous course: C3202 Prerequisite: 23110.23117/24001 Recommended semester: 3rd semester Examination: Evaluation of report(s) (Pass/fail) Participant limitation: max. 120 Contact person:  Maj-Britt Fruekilde, Building 224, Tel. +45 4525 2747, email maj-britt.fruekilde@biocentrum.dtu.dk Jan Martinussen, Building 301, Tel. +45 4525 2498, email imjm@pop.dtu.dk Department: Department of Biochemistry and Nutrition Aim: To teach basic principles of experimental work with biological matter (tissue, cells and biomolecules). Focussing also on environmentally relevant topics (the influence of toxic substances on enzyme reactions, growth, regulation, bacteriological water analysis), the course expands on the theory from lecture course 24001 in a vivid manner. Contents: The chemical composition of the cell, detection and physical chemical characterisation of biomacromolecules (DNA, RNA, protein, lipid, carbohydrate). Cell metabolism: investigation of metabolic processes and their regulation, including measurement of enzyme reactions and the influence of toxic substances (enzyme kinetics and inhibition). Microbiological techniques: sterile techniques, cell culture, isolation of industrial relevant strains from the nature, waste water analysis, and bacteria in food fermentation. DNA technology: gene cloning and purification and analysis of DNA.