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25261 Experimental Biochemistry 2
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Danish title: Eksperimentel biokemi 2
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Language: English
Credit points: 5 |
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| Type: | Ph.D.-level, Open University
Language: English |
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Previous course: C2561
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No credit points with: C2561
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Prerequisite: 25151,25111
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Desirable: 25221,25231
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Recommended semester: 5th semester
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Examination: Approval of exercises. (Pass/fail)
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Participant limitation: max. 16
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Department: Department of Biochemistry and Nutrition
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Aim: To introduce advanced methodology in biochemical research.
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Contents: Biochemical techniques: Extraction methods, preparative column chromatography, incl. FPLC and affinity-chromatography. Analytical methods for characterization of biomacromolecules, incl. gel electrophoresis, immunelectrophoresis, isoelectric focusing, ELISA-techniques, immunblotting, HPLC, FPLC and GC. Metabolic studies: Purification of subcellular organelles using preparative ultracentrifugation, use of marker enzymes. Purification of peripheral neutrophils from man and peritoneal neutrophils from rats. Application of radioisotopes in metabolic studies. Image processing and classification of complex biochemical data. Isolation, separation and characterization of lipoproteins.
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