DTU
Uddannelse
Previous page | Current version Archive 1999/2000 
 
25780 Separation Methods for Biological Compounds
Danish title: Separationsmetoder for biologiske materialer
Language: English Credit points: 5
Type: Open University
Language: English

Previous course: C2580
No credit points with: C2580
Recommended semester: 7th - 9th semester
Scope and form: Lectures and colloquia. Laboratory work two afternoons per week during the last half of the term.
Examination: Approval of compulsory activities is a prerequisite for taking part in the examination. Written exam (13-scale)
Participant limitation: max. 16

Contact person: Vibeke Barkholt, Building 224, Tel. +45 4525 2751, email vibeke.barkholt@biocentrum.dtu.dk

Department: Department of Biochemistry and Nutrition
Contents: Lectures on the theoretical basis of separation and detection systems. Theoretical and instrumental introduction to HPLC, GC, capillary electrophoresis, mass spectrometry, two dimensional electrophoresis,centrifugation, amino acid analysis, and protein sequencing. The experimental work will include sample preparation, column packing test, and evaluation of columns and separations of amino acids, proteins, vitamins, lipids and membranefractions from different types of tissue. The utility of the methods will be judged with special attention to their compatibility with the stability of biological material.