| Type: | Open University
Language: English |
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Previous course: C3005/30405
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No credit points with: C3005.30405
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Prerequisite: 30001/24101
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Recommended semester: 7th semester
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Scope and form: 2 lecture modules including problem solving per week.
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Examination: Approval of compulsory activities is a prereqisite for evaluation of report. Report. (13-scale)
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| Contact person: | Jens Nielsen, Building 223, Tel. +45 4525 2696, email jn@ibt.dtu.dk |
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Department: Department of Biotechnology
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Aim: The aim of the course is to give the students a fundamental understanding of the interplay between the many different intracellular reactions, and especially how the fluxes through the different pathways are regulated; with special focus on pathways leading to industrially relevant products like primary metabolites, antibiotics, industrial enzymes, and pharmaceutical proteins. A central aspect of the course is to identify the optimal strategy for introducing directed genetic changes in the microorganisms with the aim of obtaining better production strains.
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Contents: The course gives an overview of the different elements of metabolic engineering with a number of examples on how directed genetic modification using rDNA technology have been introduced with the aim of obtaining better strains for production of different compounds in the bioindustry. There is especially focus on the different tools of metabolic engineering, and the course covers the following topics: Introduction to metabolic engineering. Overview of biochemical pathways. Energetics. Regulation of pathways. Examples of metabolic engineering. Identification of gross measurement errors. Metabolic flux analysis: Theory and applications. Application of 13C-isotopes for quantification of metabolic fluxes. Metabolic control analysis: Theory and Application. Application of metabolic control analysis on complex reaction network. Pathway synthesis. Thermodynamic analysis of biochemical reactions. Classical and non-equilibrium thermodynamics of biochemical reactions. In the description there is especially focus on a few processes, e.g. amino acid production by Corynebacteria, production of antibiotics, lactic acid fermentation, and industrial applications of the yeast Saccharomyces cerevisiae.
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