| Type: | Open University
Language: Danish |
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Compulsory: 91531
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Recommended semester: 4th semester
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Scope and form: Classroom lectures.
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Examination: Approval of compulsory reports is a prerequisite for the exam. Written exam partly with aid. (13-scale)
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| Contact person: | Dorte Jørck-Ramberg, Building 378, Tel. +45 4525 5502, email ifakdjr@pop.dtu.dk |
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Department: Department of Applied Chemistry
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Aim: The aim of the course is to broaden the knowledge of unicellular organisms structure, morphology and physiology in addition to the work with biological materials and systems. To give a knowledge of Good Laboratory Practices (GLP) and Good Manufacturing Practices (GMP) for microbiological and biochemical work. To obtain general skills of biomethodology.
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Contents: Biochemical part : the synthesis and control of synthesis of monomers and macromolecules, e.g. the protein synthesis. Enzymes kinetics. Microbiological part : Techniques of culturing, analytical use of microorganisms, fermentation kinetics and -techniques (batch and chemostat). Sterilization kinetics and techniques. Biomethodology : GLP/GMP for biological laboratories. Separation techniques for biological molecules. Chromatograhic and electrophoretic techniques. Enzymatic and immunological techniques. Methods of detection for different groups of molecules. Litterature critics.
The practical part of the course contains : Microbiology : Qualitative and quantitative detection of microorganisms. Microbiological assays. Fermentations. Biochemistry : Enzymatic analysis (end-point and kinetics). Purification of enzymes, e.g. by precipitation, chromatography and electrophoresis. Characterization of enzymes. Immunology. Analytical characterization of specified products.
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